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M9490012.TXT
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1994-09-03
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Document 0012
DOCN M9490012
TI [Diagnosis of FIV infection]
DT 9411
AU Hartmann K; Kuffer M; Egberink H; Lutz H; Kraft W; I. Medizinischen
Tierklinik, Ludwig-Maximilians-Universitat; Munchen.
SO Tierarztl Prax. 1994 Jun;22(3):268-72. Unique Identifier : AIDSLINE
MED/94323946
AB The FIV PetCheck ELISA is a practical and commercially available method
routinely used for the detection of anti-FIV antibodies in FIV
infection. However, false positive and false negative results occur,
although the reliability of the negative results is higher than that of
the positive results. To determine the predictive value of a positive
result in Bavaria, 1911 randomly selected cat sera were collected. Using
the PetCheck anti-FIV ELISA, 103 (5.4%) of the 1911 samples tested were
positive. These positive samples were reexamined by two other ELISA and
by Western Blot. Sixty-one (59.2%) were positive with the second ELISA,
53 (51.5%) with the third ELISA and 40 (40.7%) by Western Blot.
Therefore, 59.2% of all positive samples could be considered false
positive. The ELISA as a single method to diagnose FIV infection is not
reliable in Germany due to the low prevalence of FIV infection.
Therefore, a Western Blot confirmation of every positive ELISA is of
absolute necessity.
DE Animal Antibodies, Viral/*BLOOD Blotting, Western/*VETERINARY Cats
English Abstract Enzyme-Linked Immunosorbent Assay/*VETERINARY False
Negative Reactions False Positive Reactions Feline Acquired
Immunodeficiency Syndrome/*DIAGNOSIS/EPIDEMIOLOGY Germany/EPIDEMIOLOGY
Immunodeficiency Virus, Feline/*IMMUNOLOGY Predictive Value of Tests
Prevalence JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).